ISSN: 1598-3579
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IJIE, vol. 34, no. 1, pp.1-5, March, 2017

DOI: http://dx.doi.org/10.7852/ijie.2017.34.1.1

Expression of Recombinant Human Bone Morphogenic protein 2 (hBMP2) in Insect cells

Seong-Wan Kim, Seong-Ryul Kim, Seung Won Park, Tae-Won Goo, Kwang-Ho Choi
Tae-Won Goo, Dept Biochem, Dongguk University Kwang-Ho Choi, Dept Agricul Biol, Nat Inst Agric Sci

Abstract: Bone morphogenetic protein 2 (BMP2) plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is involved also in cardiac cell differentiation and epithelial to mesenchymal transition. In this study, We expressed human BMP2 (hBMP2) recombinant protein using Baculovirus Expression Vector System (BEVS) in Sf9 insect cells. The hBMP2 cDNA was cloned into baculovirus transfer vector, pBacgus-4x-1 and recombinant baculovirus was screened out through X-gal and GUS-fusions assay. Western blot analysis shown that molecular weight of hBMP2 recombinant protein was about 44.71 kDa.

Keyword: Bombyx mori, BMP2, Baculovirus, Silkworm, recombinant protein

 

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