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IJIE, vol. 34, no. 1, pp.6-10, March, 2017

DOI: http://dx.doi.org/10.7852/ijie.2017.34.1.6

Dissolution of degummed Antheraea yamamai silkworm cocoon

You-Young Jo, Sung Min Bae, HaeYong Kweon*
HaeYong Kweon, Natl Inst Agric Sci

Abstract: Dissolution of Antheraea yamamai silkworm cocoon was carried out in various solvent systems with various dissolving conditions including dissolution salts, salt concentration, dissolving temperature, and time. General chaotropic salt for Bombyx mori silk fibroin does not work for A. yamamai silkworm cocoon. Lithium bromide 9.3 M at 100oC also does not work to dissolve wild silkworm cocoon. However, 9 M of lithium thiocyanate treatment at 100oC induced 100% dissolution of wild silkworm cocoon. But it could not be dissolved lower than 60oC. Like lithium thiocyanate, less than 60oC treatment with molten calcium nitrate 4 hydrate could not dissolve wild silkworm cocoon. As the dissolution temperature increased up to 100oC, the solubility of wild one was reached over 90%. SDS-PAGE showed broad tailing stream pattern that means the molecule of wild silk was depolymerized with dissolution temperature and time. From the above results, the best chaotropic salt for A.yamamai silkworm cocoon is calcium nitrate 4 hydrate.

Keyword: Antheraea yamamai silk, Dissolution, Calcium nitrate, Molecular weight degradation

 

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