ISSN: 1598-3579
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IJIE, vol. 35, no. 1, pp.7-13, January, 2017


Molecular weight-associated cellular response to silk fibroin fragments demonstrated in MG63 cells

You-Young Jo, HaeYong Kweon, Seong-Gon Kim
National Institute of Agricultural Science Gangneung-Wonju National Univeristy

Abstract: In this study, changes in gene expression after administration of silk fibroin fragments (size ? 30 kDa) were evaluated in MG63 cells using a cDNA microarray assay. In addition, the level of alkaline phosphatase (ALP) activity and cellular proliferation in the group administered moderately sized silk fibroin fragments (size ? 30 kDa) (MSF) were compared to those in the group administered smaller silk fibroin fragments (size < 1 kDa) (SSF). The results of the cDNA microarray assay show increased expression of genes that are related to the cell cycle and inflammation. ALP, bone morphogenetic protein-7, bone morphogenetic protein receptor type IA, and runt-related transcription factor 2 exhibited significantly lower expression compared to control cells (fold ratio < 0.5). Relative ALP activity of the 100 μg/mL MSF group was significantly lower than that of the SSF group (P < 0.05). Thus, the MSF group showed increased expression of genes associated with cellular proliferation and inflammation but decreased expression of genes associated with osteogenesis.

Keyword: silk fibroin, cDNA microarray, MG63 cells, alkaline phosphatase


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